Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes

0518362 - BFÚ 2020 RIV CH eng J - Journal Article
Hasoň, Stanislav - Fojta, Miroslav - Ostatná, Veronika
Label-free electrochemical analysis of purine nucleotides and nucleobases at disposable carbon electrodes in microliter volumes.
Journal of Electroanalytical Chemistry. Roč. 847, AUG 15 2019 (2019), č. článku 113252. ISSN 1572-6657. E-ISSN 1873-2569
R&D Projects: GA ČR(CZ) GA17-08971S; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707
Keywords : dna bases * pencil electrodes * graphene * graphite
OECD category: Analytical chemistry
Impact factor: 3.807, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S157266571930520X?via%3Dihub

We analyzed purine nucleotides in oligodeoxynucleotides (ODN) and nucleobase residues acid-hydrolyzed ODN at nanomolar concentrations in few tens of microliters of solution at commercially available unmodified graphite pencil electrodes, screen printed graphite electrodes (SPGEs) and SPGEs modified by graphene, single- and multi-walled carbon nanotubes. Our results suggest that specific properties of different graphite-based electrodes have significant impact on ODN analysis. Nanostructure-modified electrodes produced higher signals due to purine nucleobase oxidation but also higher background currents. The unmodified disposable carbon electrodes allowed analysis of both free nucleobases and ODNs. Our results show that unmodified SPGEs represent a good compromise for signal/noise ratio. Electrooxidation of purines at these electrodes was only little affected by the presence of pyrimidines in contrast to the pencil graphite electrode.
Permanent Link: http://hdl.handle.net/11104/0303516