Rapid screening of very long-chain fatty acids from microorganisms

0518107 - MBÚ 2020 RIV NL eng J - Journal Article
Řezanka, Tomáš - Vítová, Milada - Lukavský, Jaromír - Nedbalová, L. - Kolouchová, I.
Rapid screening of very long-chain fatty acids from microorganisms.
Journal of Chromatography A. Roč. 1605, NOV 8 2019 (2019), č. článku UNSP 460365. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971 ; RVO:67985939
Keywords : High resolution tandem mass spectrometr * Very long-chain fatty acids * GC-MS
OECD category: Microbiology
Impact factor: 4.049, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0021967319307496?via%3Dihub

The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:19, 1 omega 9, 24:6 omega 3, 26:0, 26:1 omega 9, 28:0, 28:1 omega 9, 28:2 omega 6 and 28:8 omega 3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (R1COO- and R2COO-). The limit of detection was determined at 10 nmol/L (similar to 11 pg/mu L) in triacylglycerols and 8.6 nmoles/L (similar to 8 pg/mu L) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids
Permanent Link: http://hdl.handle.net/11104/0303295