pH-responsive and antibacterial properties of self-assembled multilayer films based on chitosan and tannic acid

0518031 - ÚMCH 2021 RIV NL eng J - Journal Article
Kumorek, Marta M. - Minisy, Islam M. - Krunclová, Tereza - Voršiláková, Marta - Venclíková, Kristýna - Mázl Chánová, Eliška - Janoušková, Olga - Kubies, Dana
pH-responsive and antibacterial properties of self-assembled multilayer films based on chitosan and tannic acid.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 109, April (2020), s. 1-13, č. článku 110493. ISSN 0928-4931. E-ISSN 1873-0191
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LQ1604; GA MZd(CZ) NV16-28254A
Institutional support: RVO:61389013
Keywords : tannic acid * chitosan * layer-by-layer
OECD category: Polymer science
Impact factor: 7.328, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0928493119303339?via%3Dihub

Polyelectrolyte layer-by-layer (LbL) films that disintegrate under physiological conditions are intensively studied as coatings to enable the release of bioactive components. Herein, we report on the interactions and pH-stability of LbL films composed of chitosan (CH) or N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (CMCH) and tannic acid (TA), employed to guarantee the film disintegration. The self-assembly of TA with CH and CMCH at pH 5 and with CMCH at pH 7.4 were proven by turbidimetric, surface plasmon resonance and UV–Vis analyses. The LbL films exhibited pH-dependent properties. CMCH/TA films prepared at pH 7.4 showed exponential growth as well as a higher layer thickness and surface roughness, whereas films prepared at pH 5 grew linearly and were smoother. The film stability varied with the pH used for film assembly. CH/TA films assembled at pH 5 were unstable at pH 8.5, whereas CMCH/TA films assembled at pH 7.4 disintegrated at pH 4. All films exhibited a similar disassembly at pH 7.4. The coatings reduced the adhesion of E. coli and S. aureus by approximately 80%. CMCH-terminated CMCH/TA films were more resistant to bacterial adhesion, whereas CH-terminated CH/TA films demonstrated stronger killing activity. The prepared pH-triggered decomposable LbL films could be used as degradable coatings that allow the release of therapeutics for biomedical applications and also prevent bacterial adhesion.
Permanent Link: http://hdl.handle.net/11104/0304000