Infrared absorption spectroscopy of albumin binding with amine-containing plasma polymer coatings on nanoporous diamond surfaces

0517868 - FZÚ 2020 RIV US eng J - Journal Article
Kozak, Halyna - Artemenko, Anna - Ukraintsev, Egor - Choukurov, A. - Rezek, Bohuslav - Kromka, Alexander
Infrared absorption spectroscopy of albumin binding with amine-containing plasma polymer coatings on nanoporous diamond surfaces.
Langmuir. Roč. 35, č. 43 (2019), s. 13844-13852. ISSN 0743-7463
R&D Projects: GA MŠMT(CZ) EF16_019/0000760; GA ČR(CZ) GA17-19968S
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : protein * diamond * XPRS * GAR-FTIR
OECD category: Biophysics
Impact factor: 3.557, year: 2019
Method of publishing: Limited access
https://doi.org/10.1021/acs.langmuir.9b02327

Nanocrystalline diamond (NCD) layers functionalized with amine-containing functional groups have generated considerable interest as biocompatible substrates for attachment of biomolecules and cells. Here we prepare nanoporous diamond layers with the surfaces modified by H, O plasma, and conformal 7 nm amine-containing plasma polymer (PP). Immobilization of bovine serum albumin (BSA) molecules is characterized on such surfaces. GAR-FTIR as well as XPS show that concentration of amine-containing bonds after BSA exposure depends on the type of NCD surface modification. AFM measurements reveal that BSA proteins are physisorbed on H- and O-terminated diamond surfaces in different thicknesses and morphology. When the diamond layers are coated with the amine-containing PP, BSA molecules assume similar thickness and morphology, and their adhesion is significantly increased on both types of the diamond surfaces.

Permanent Link: http://hdl.handle.net/11104/0303125