Features of the morphofunctional state of parotid salivary glands in six-month-old rats with experimentally induced fetal macrosomia

0517536 - FGÚ 2020 RIV UA rus J - Journal Article
Garmash, O. V. - Gubina-Vakulik, H. I. - Vondrášek, David
Features of the morphofunctional state of parotid salivary glands in six-month-old rats with experimentally induced fetal macrosomia.
[Osoblivosti morfofunkcional‘nogo stanu privušnich slinnich zaloz šestimisjačnich ščuriv pri eksperimental‘no model‘ovanij vnutrišn‘outrobnij makrosomii.]
Pathologia. Roč. 1, Jan-Apr (2019), s. 106-115. ISSN 2306-8027. E-ISSN 2310-1237
R&D Projects: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Research Infrastructure: Czech-BioImaging - 90062
Institutional support: RVO:67985823
Keywords : fetal macrosomia * experimental animal models * parotid gland
OECD category: Cell biology
Method of publishing: Open access
http://pat.zsmu.edu.ua/article/view/166469

The aim of the study is to examine experimentally the features of postnatal morphogenesis of parotid salivary glands in six-month-old rats with fetal macrosomia and compare the morphofunctional condition of parotid glands between two groups of 6-month-old and 3-month-old rats with induced macrosomia.
Four macrosomia models have been used by applying a high-calorie diet and hypokinesia to pregnant Wistar rats. After birth, the descendant rats have been weighed and divided into groups taking into account their calculated weight-length parameters. Four main groups and a comparison group have been created. The main (macrosomic) groups consist of species with the following parameters: (1) long body length and relatively decreased body weight, (2) balanced intrauterine development, (3) standard body length and intrauterine obesity, (4) standard body weight and intrauterine obesity induced by hypokinesia of a pregnant female rat. At the age of 6 months, the rats have been sacrificed. The absolute and relative weights of parotid glands have been evaluated.
A histopathologic analysis revealing DNA and RNA (Gallocyanin-chrome alum technique) has been carried out after coating the parotid glands with paraffin, the PAS-reaction has been used to reveal glycoproteins. The serocytes nuclei cross section, the content of DNA in the cell nucleus, and the content of RNA in cytoplasm have been measured using computer images. Using the images obtained with confocal laser scanning microscopy, the gland acini cross section and the acini cross section perimeter of the parotid gland have been measured. The same technique has been used to measure the serocyte cross section. Caspase-3 immunehistochemical staining has been performed.
Comparison of the morphofunctional condition of the parotid gland at the age of 3 and 6 months has revealed that the age-specific enlargement of the parotid gland is less in the rats born with macrosomia and with both body mass deficiency and obesity, than that in the comparison group. The rats born with signs of body mass deficiency show an economic type of gland functioning, whereas the animals born with intrauterine obesity show signs of parotid gland atrophy with compensatory morphofunctional activation of functioning serocytes.
The somatometric features of fetal macrosomia affect the morphofunctional features of the parotid gland of descendant animals at the age of 6 months (mature age).


The aim of the study is to examine experimentally the features of postnatal morphogenesis of parotid salivary glands in six-month-old rats with fetal macrosomia and compare the morphofunctional condition of parotid glands between two groups of 6-month-old and 3-month-old rats with induced macrosomia.
Four macrosomia models have been used by applying a high-calorie diet and hypokinesia to pregnant Wistar rats. After birth, the descendant rats have been weighed and divided into groups taking into account their calculated weight-length parameters. Four main groups and a comparison group have been created. The main (macrosomic) groups consist of species with the following parameters: (1) long body length and relatively decreased body weight, (2) balanced intrauterine development, (3) standard body length and intrauterine obesity, (4) standard body weight and intrauterine obesity induced by hypokinesia of a pregnant female rat. At the age of 6 months, the rats have been sacrificed. The absolute and relative weights of parotid glands have been evaluated.
A histopathologic analysis revealing DNA and RNA (Gallocyanin-chrome alum technique) has been carried out after coating the parotid glands with paraffin, the PAS-reaction has been used to reveal glycoproteins. The serocytes nuclei cross section, the content of DNA in the cell nucleus, and the content of RNA in cytoplasm have been measured using computer images. Using the images obtained with confocal laser scanning microscopy, the gland acini cross section and the acini cross section perimeter of the parotid gland have been measured. The same technique has been used to measure the serocyte cross section. Caspase-3 immunehistochemical staining has been performed.
Comparison of the morphofunctional condition of the parotid gland at the age of 3 and 6 months has revealed that the age-specific enlargement of the parotid gland is less in the rats born with macrosomia and with both body mass deficiency and obesity, than that in the comparison group. The rats born with signs of body mass deficiency show an economic type of gland functioning, whereas the animals born with intrauterine obesity show signs of parotid gland atrophy with compensatory morphofunctional activation of functioning serocytes.
The somatometric features of fetal macrosomia affect the morphofunctional features of the parotid gland of descendant animals at the age of 6 months (mature age).
Permanent Link: http://hdl.handle.net/11104/0302877