Ganoderma Lucidum induces oxidative DNA damage and enhances the effect of 5-Fluorouracil in colorectal cancer in vitro and in vivo

0517497 - ÚEM 2020 RIV NL eng J - Journal Article
Opattová, Alena - Horák, Josef - Vodenková, Soňa - Kostovčíková, Klára - Čumová, Andrea - Macinga, P. - Galanová, Natálie - Rejhová, Alexandra - Vodičková, Ludmila - Kozicz, K. - Turnovcová, Karolína - Hucl, T. - Slíva, Daniel - Vodička, Pavel
Ganoderma Lucidum induces oxidative DNA damage and enhances the effect of 5-Fluorouracil in colorectal cancer in vitro and in vivo.
Mutation Research - Genetic Toxicology and Environmental Mutagenesis. Roč. 845, SI (2019), č. článku UNSP 403065. ISSN 1383-5718. E-ISSN 1879-3592
R&D Projects: GA ČR(CZ) GA18-09709S; GA ČR(CZ) GA19-10543S; GA MZd(CZ) NV15-27580A
Institutional support: RVO:68378041 ; RVO:61388971
Keywords : ganoderma lucidum * colorectal cancer * DNA damage
OECD category: Cell biology; Immunology (MBU-M)
Impact factor: 2.506, year: 2019
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S1383571819300944?via%3Dihub

The first-line chemotherapy of colorectal cancer (CRC), besides surgery, comprises administration of 5-Fluorouracil (5FU). Apart from cytotoxic effect on cancer cells, 5FU may also cause adverse side effects. Ganoderma Lucidum (GLC) is a mushroom used in Traditional Eastern Medicine. We propose that natural compounds, particularly GLC extracts, may sensitize cancer cells to conventional chemotherapeutics. This combination therapy could lead to more selective cancer cell death and may improve the response to the therapy and diminish the adverse effects of anticancer drugs.

Here we demonstrate that GLC induced oxidative DNA damage selectively in colorectal cancer cell lines, whereas it protected non-malignant cells from the accumulation of reactive oxygen species. Accumulation of DNA damage caused sensitization of cancer cells to 5FU resulting in improved anticancer effect of 5FU. The results obtained in colorectal cell lines were confirmed in in vivo study: GLC co-treatment with 5FU increased the survival of treated mice and reduced the tumor volume in comparison with group treated with 5FU alone.

Combination of conventional chemotherapeutics and natural compounds is a promising approach, which may reduce the effective curative dose of anticancer drugs, suppress their adverse effects and ultimately lead to better quality of life of CRC patients.
Permanent Link: http://hdl.handle.net/11104/0302836