Brassinosteroid signaling delimits root gravitropism via sorting of the Arabidopsis PIN2 auxin transporter.

0517430 - ÚEB 2020 RIV GB eng J - Journal Article
Retzer, Katarzyna - Akhmanova, A. - Konstantinova, N. - Malínská, Kateřina - Leitner, J. - Petrášek, Jan - Luschnig, C.
Brassinosteroid signaling delimits root gravitropism via sorting of the Arabidopsis PIN2 auxin transporter.
Nature Communications. Roč. 10, č. 1 (2019), č. článku 5516. E-ISSN 2041-1723
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA ČR(CZ) GJ19-13375Y; GA MŠMT(CZ) EF16_013/0001775
Grant - others:AV ČR(CZ) MSM200381701; OPPK(XE) CZ.2.16/3.1.00/21519
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:61389030
Keywords : auxin signaling * PIN2 * brassinolide * gravistimulated roots
OECD category: Biochemical research methods
Impact factor: 12.121, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1038/s41467-019-13543-1

Arabidopsis PIN2 protein directs transport of the phytohormone auxin from the root tip into the root elongation zone. Variation in hormone transport, which depends on a delicate interplay between PIN2 sorting to and from polar plasma membrane domains, determines root growth. By employing a constitutively degraded version of PIN2, we identify brassinolides as antagonists of PIN2 endocytosis. This response does not require de novo protein synthesis, but involves early events in canonical brassinolide signaling. Brassinolide-controlled adjustments in PIN2 sorting and intracellular distribution governs formation of a lateral PIN2 gradient in gravistimulated roots, coinciding with adjustments in auxin signaling and directional root growth. Strikingly, simulations indicate that PIN2 gradient formation is no prerequisite for root bending but rather dampens asymmetric auxin flow and signaling. Crosstalk between brassinolide signaling and endocytic PIN2 sorting, thus, appears essential for determining the rate of gravity-induced root curvature via attenuation of differential cell elongation.
Permanent Link: http://hdl.handle.net/11104/0302738