0511984 - FGÚ 2020 RIV GB eng J - Journal Article
Valenti, D. - Neves, J. F. - Cantrelle, F. X. - Hristeva, S. - Santo Lentini, D. - Obšil, Tomáš - Hanoulle, X. - Levy, L. M. - Tzalis, D. - Landrieu, I. - Ottmann, Ch.
Set-up and screening of a fragment library targeting the 14-3-3 protein interface.
MedChemComm. Roč. 10, č. 10 (2019), s. 1796-1802. ISSN 2040-2503. E-ISSN 2040-2511
Institutional support: RVO:67985823
Keywords : 14-3-3 protein * protein-protein interactions * structure * fragment screening * nuclear magnetic resonance
OECD category: Biochemistry and molecular biology
Impact factor: 2.807, year: 2019
Method of publishing: Limited access
https://pubs.rsc.org/en/content/articlelanding/2019/MD/C9MD00215D#!divAbstract

Protein-protein interactions (PPIs) are at the core of regulation mechanisms in biological systems and consequently became an attractive target for therapeutic intervention. PPIs involving the adapter protein 14-3-3 are representative examples given the broad range of partner proteins forming a complex with one of its seven human isoforms. Given the challenges represented by the nature of these interactions, fragment-based approaches offer a valid alternative for the development of PPI modulators. After having assembled a fragment set tailored on PPIs' modulation, we started a screening campaign on the sigma isoform of 14-3-3 adapter proteins. Through the use of both mono- and bi-dimensional nuclear magnetic resonance spectroscopy measurements, coupled with differential scanning fluorimetry, three fragment hits were identified. These molecules bind the protein at two different regions distant from the usual binding groove highlighting new possibilities for selective modulation of 14-3-3 complexes.
Permanent Link: http://hdl.handle.net/11104/0302214