Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs

0511690 - BFÚ 2020 RIV DE eng J - Journal Article
Římánková, Ludmila - Ostatná, Veronika - Bartošík, M.
Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs.
Electroanalysis. Roč. 31, č. 10 (2019), s. 1895-1900. ISSN 1040-0397. E-ISSN 1521-4109
Institutional support: RVO:68081707
Keywords : signal amplification strategy * electrochemical determination * stripping voltammetry * cyclic voltammetry * polarography * chitosan * microRNA
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 2.544, year: 2019
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201900241

We show for the first time that RNA catalyzes hydrogen evolution reaction at mercury-containing electrodes. We previously showed that DNA is electrocatalytically active, and so we compared heights and potentials of the RNA chronopotentimetric stripping (CPS) peaks with analogous signals of DNA of the same sequence and found out they were very similar. RNA peaks showed differences depending on the RNA base composition. Catalytic nature of CPS peak enabled detection of 25 pM microRNA in electrochemical cell, or 500 pM microRNA in a 5 mu L solution drop (corresponding to 2.5 fmole of microRNA). This finding opens the door for simple, label-free and reagent-less analysis of low concentrations of RNA molecules.
Permanent Link: http://hdl.handle.net/11104/0301909