Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces

0511604 - ÚIACH 2021 RIV CZ eng C - Conference Paper (international conference)
Hlaváček, Antonín - Křivánková, Jana - Foret, František
Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces.
NANOCON Conference Proceedings - International Conference on Nanomaterials. Volume 2020-October. Ostrava: Tanger Ltd., 2020, s. 317-322. 1st Edition. ISBN 978-808729495-6. ISSN 2694-930X.
[NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./. Brno (CZ), 16.10.2019-18.10.2019]
R&D Projects: GA ČR(CZ) GJ18-03367Y
Institutional support: RVO:68081715
Keywords : photon-upconversion * nanoparticle * laser scanner * hyperspectral imaging
OECD category: Analytical chemistry
https://www.confer.cz/nanocon/2019

Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
Permanent Link: http://hdl.handle.net/11104/0311152