An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye

0510838 - ÚMG 2020 RIV GB eng J - Journal Article
Nguyen, M.T.T. - Vemaraju, S. - Nayak, G. - Odaka, Y. - Buhr, E. D. - Alonzo, N. - Tran, U. - Batie, M. - Upton, B. A. - Darvas, M. - Kozmik, Zbyněk - Rao, S. - Hegde, R. S. - Iuvone, P. M. - Van Gelder, R. N. - Lang, R.A.
An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye.
Nature Cell Biology. Roč. 21, č. 4 (2019), s. 420-429. ISSN 1465-7392. E-ISSN 1476-4679
R&D Projects: GA MŠMT(CZ) ED1.100/02.0109
Institutional support: RVO:68378050
Keywords : retinal dopamine * ganglion-cells * mammalian retina * rodent retina * mouse * melanopsin * phosphorylation * retinopathy * opn5 * photoentrainment
OECD category: Cell biology
Impact factor: 20.042, year: 2019
Method of publishing: Limited access
https://www.nature.com/articles/s41556-019-0301-x

During mouse postnatal eye development, the embryonic hyaloid vascular network regresses from the vitreous as an adaption for high-acuity vision. This process occurs with precisely controlled timing. Here, we show that opsin 5 (OPN5, also known as neuropsin)-dependent retinal light responses regulate vascular development in the postnatal eye. In Opn5-null mice, hyaloid vessels regress precociously. We demonstrate that 380-nm light stimulation via OPN5 and VGAT (the vesicular GABA/glycine transporter) in retinal ganglion cells enhances the activity of inner retinal DAT (also known as SLC6A3, a dopamine reuptake transporter) and thus suppresses vitreal dopamine. In turn, dopamine acts directly on hyaloid vascular endothelial cells to suppress the activity of vascular endothelial growth factor receptor 2 (VEGFR2) and promote hyaloid vessel regression. With OPN5 loss of function, the vitreous dopamine level is elevated and results in premature hyaloid regression. These investigations identify violet light as a developmental timing cue that, via an OPN5-dopamine pathway, regulates optic axis clearance in preparation for visual function.
Permanent Link: http://hdl.handle.net/11104/0306731