Fluorescent Probes for Monitoring Cholesterol Trafficking in Cells

0510437 - ÚMG 2020 RIV CZ eng J - Journal Article
Králová, Jarmila - Král, V.
Fluorescent Probes for Monitoring Cholesterol Trafficking in Cells.
Folia Biologica. Roč. 65, č. 1 (2019), s. 1-10. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA MŠMT LO1220; GA ČR GA17-02836S
Institutional support: RVO:68378050
Keywords : nonvesicular sterol transport * bodipy-cholesterol * living cells * membrane cholesterol * perfringolysin-o * lipid-bilayers * rich domains * analogs * dehydroergosterol * filipin
OECD category: Cell biology
Impact factor: 0.691, year: 2019
Method of publishing: Open access
https://fb.cuni.cz/volume-65-2019-no-1#articFB2019A0001

Cellular cholesterol plays fundamental and diverse roles in many biological processes and affects the pathology of various diseases. Comprehensive and detailed understanding of the cellular functions and characteristics of cholesterol requires visualization of its subcellular distribution, which can be achieved by fluorescence microscopy. Many attempts have been made to develop fluorescent cholesterol reporters, but so far, none of them seems to be ideal for studying all aspects of cholesterol management. To meet the requirements for the right probe remains a great challenge, and progress in this field continues. The main objective of this review is to not only present the current state of the art, but also critically evaluate the applicability of individual probes and for what purpose they can be used to obtain relevant data. Hence, the data obtained with different probes might provide complementary information to build an integrated picture about the cellular cholesterol.
Permanent Link: http://hdl.handle.net/11104/0306734