Down-regulation of vimentin by triorganotin isothiocyanates—nuclear retinoid X receptor agonists: A proteomic approach

0510284 - ÚIACH 2021 RIV IE eng J - Journal Article
Strouhalová, Dana - Macejová, D. - Mosná, B. - Bobál, P. - Otevřel, J. - Laštovičková, Markéta - Brtko, J. - Bobálová, Janette
Down-regulation of vimentin by triorganotin isothiocyanates—nuclear retinoid X receptor agonists: A proteomic approach.
Toxicology Letters. Roč. 318, JAN (2020), s. 22-29. ISSN 0378-4274. E-ISSN 1879-3169
Grant - others:AV ČR(CZ) SAV-18-16
Program: Bilaterální spolupráce
Institutional support: RVO:68081715
Keywords : triorganotin isothiocyanates * breast cancer * proteomic
OECD category: Analytical chemistry
Impact factor: 4.374, year: 2020
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0378427419303236?via%3Dihub

An attempt has been made to delineate the role of natural and synthetic retinoid receptor ligands on vimentin expression in the human triple-negative breast cancer cells. The effects of currently synthesized triorganotin
derivatives of the general formula R3SnX (R is butyl or phenyl, X is isothiocyanate), which are considered RXR igands, were investigated in the human MDA-MB-231 breast cancer cell line. Studies were evaluated in the
presence and absence of all-trans retinoic acid (ATRA), a natural RAR ligand. Vimentin represents the major protein associated with epithelial-mesenchymal transition (EMT), an essential process when the primary tumour
transforms into a malignant one. mRNA and proteomic data obtained in this study, based on the PDQuest software protein evaluation and further quantification of proteins by iTRAQ analysis, suggest that vimentin was
significantly reduced in the combination of RAR ligand and RXR ligand treatment. Both tested triorganotin compounds showed similarly reduced expression of vimentin, but tributyltin isothiocyanate (TBT-ITC) proved to
be more effective than triphenyltin isothiocyanate (TPT-ITC). Furthermore, the effect of natural (9cRA) and synthetic RXR ligands, both chloride and isothiocyanate derivatives, on vimentin expression was compared.
Permanent Link: http://hdl.handle.net/11104/0300800