The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria

0510253 - BFÚ 2020 RIV CH eng J - Journal Article
Bartas, M. - Cutova, M. - Brázda, Václav - Kaura, P. - Šťastný, J. - Kolomaznik, J. - Coufal, Jan - Goswami, Pratik - Cerven, J. - Pečinka, P.
The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria.
Molecules. Roč. 24, č. 9 (2019). E-ISSN 1420-3049
R&D Projects: GA ČR(CZ) GA18-15548S
Institutional support: RVO:68081707
Keywords : genome-wide * rna quadruplex * dna * motifs * radiodurans * prediction * promoters
OECD category: Biochemistry and molecular biology
Impact factor: 3.267, year: 2019
Method of publishing: Open access
https://www.mdpi.com/1420-3049/24/9/1711/pdf

The role of local DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, the significance of G-quadruplexes was demonstrated in the last decade, and their presence and functional relevance has been demonstrated in many genomes, including humans. In this study, we analyzed the presence and locations of G-quadruplex-forming sequences by G4Hunter in all complete bacterial genomes available in the NCBI database. G-quadruplex-forming sequences were identified in all species, however the frequency differed significantly across evolutionary groups. The highest frequency of G-quadruplex forming sequences was detected in the subgroup Deinococcus-Thermus, and the lowest frequency in Thermotogae. G-quadruplex forming sequences are non-randomly distributed and are favored in various evolutionary groups. G-quadruplex-forming sequences are enriched in ncRNA segments followed by mRNAs. Analyses of surrounding sequences showed G-quadruplex-forming sequences around tRNA and regulatory sequences. These data point to the unique and non-random localization of G-quadruplex-forming sequences in bacterial genomes.
Permanent Link: http://hdl.handle.net/11104/0300772