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Non-Natural Nucleic Acids: Methods and Protocols
- 1.0509903 - ÚOCHB 2020 RIV US eng M - Monography Chapter
Ménová, Petra - Cahová, Hana - Vrábel, Milan - Hocek, Michal
Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA.
Non-Natural Nucleic Acids: Methods and Protocols. New York: Humana Press, 2019 - (Shank, N.), s. 39-57. Methods in Molecular Biology, 1973. ISBN 978-1-4939-9215-7
R&D Projects: GA ČR(CZ) GA18-03305S; GA MŠMT(CZ) EF16_019/0000729
Grant - others:AV ČR(CZ) AP1501
Program: Akademická prémie - Praemium Academiae
Institutional support: RVO:61388963
Keywords : nucleotides * nucleoside triphosphates * DNA polymerases * enzymatic synthesis * modified DNA
OECD category: Organic chemistry
Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR.
Permanent Link: http://hdl.handle.net/11104/0300492
Number of the records: 1