MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

0509780 - BFÚ 2020 RIV US eng J - Journal Article
Štefková, K. - Hanackova, M. - Kučera, J. - Radaszkiewicz, K. A. - Ambrůzová, Barbora - Kubala, Lukáš - Pacherník, J.
MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells.
Stem Cells International. Roč. 2019, Č. 2019 (2019), č. článku 5128135. ISSN 1687-966X. E-ISSN 1687-9678
Institutional support: RVO:68081707
Keywords : c/ebp
OECD category: Cell biology
Impact factor: 3.869, year: 2019
Method of publishing: Open access
http://downloads.hindawi.com/journals/sci/2019/5128135.pdf

The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.
Permanent Link: http://hdl.handle.net/11104/0300408