Survival and long-term infectivity of arbuscular mycorrhizal fungi in peatbased substrates stored under different temperature regimes

0509699 - BÚ 2020 RIV NL eng J - Journal Article
Püschel, David - Kolaříková, Zuzana - Šmilauer, P. - Rydlová, Jana
Survival and long-term infectivity of arbuscular mycorrhizal fungi in peatbased substrates stored under different temperature regimes.
Applied Soil Ecology. Roč. 140, AUG 2019 (2019), s. 98-107. ISSN 0929-1393. E-ISSN 1873-0272
R&D Projects: GA MŠMT 1M0571; GA ČR(CZ) GA17-12166S
Institutional support: RVO:67985939
Keywords : arbuscular mycorrhizal fungi * storage in peat-based substrates * temperature regimes
OECD category: Ecology
Impact factor: 3.187, year: 2019
Method of publishing: Limited access

The study tested long-term infectivity of arbuscular mycorrhizal fungi (AMF) when introduced to a peat-based substrate and stored in five different temperature regimes (constant temperatures of −20, 5 or 20 °C) and two outdoor regimes simulating actual practice (shade and direct sunlight). Eight AMF inocula were tested, including monocultures of Claroideoglomus claroideum, Funneliformis caledonium, and F. mosseae plus two isolates of Rhizophagus irregularis, all applied as 4% (v) of the substrate, or their mixture in 1%, 4% and 8% (v) doses. Their infectivity was monitored for 56 weeks at 8-week intervals as measured by mycorrhizal root colonization of bioassay plants. In general, infectivity significantly decreased with time, but in three regimes including periods of cold (5 °C, shade and sunlight), cold stratification probably broke spore dormancy and led to temporal stimulation of AMF infectivity. Except for storage at 5 °C, where a wider AMF spectrum maintained sufficient long-term viability, infectivity became very low after 1 year. Temperature fluctuations in direct sunlight were less negative than expected, whereas infectivity decline was surprisingly strong also in mild storage conditions (20 °C). Higher inoculum dose lengthened AMF infectivity. R. irregularis isolates were found most resistant among the tested AMF. We ascribe the general decline of infectivity to unfavorable properties inherent to peat.
Permanent Link: http://hdl.handle.net/11104/0301566