Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana

0509662 - ÚEB 2020 RIV GB eng J - Journal Article
Syslová, Eliška - Landa, Přemysl - Navrátilová, M. - Stuchlíková Raisová, L. - Matoušková, P. - Skálová, L. - Szotáková, B. - Vaněk, Tomáš - Podlipná, Radka
Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana.
Chemosphere. Roč. 234, NOV (2019), s. 528-535. ISSN 0045-6535. E-ISSN 1879-1298
R&D Projects: GA ČR(CZ) GA18-08452S; GA ČR(CZ) GA18-07724S
Institutional support: RVO:61389030
Keywords : Arabidopsis thaliana * Ivermectin * Metabolites * Transcriptomic response * Veterinary drug
OECD category: Pharmacology and pharmacy
Impact factor: 5.778, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1016/j.chemosphere.2019.06.102

Veterinary drugs enter the environment in many ways and may affect non-target organisms, including plants. The present project was focused on the biotransformation of ivermectin (IVM), one of the mostly used anthelmintics, in the model plant Arabidopsis thaliana. Our results certified the ability of plants to uptake IVM by roots and translocate it to the aboveground parts. Using UHPLC-MS/MS, six metabolites in roots and only the parent drug in rosettes were found after 24- and 72-h incubation of A. thaliana with IVM. The metabolites were formed only via hydroxylation and demethylation, with no IVM conjugates detected. Although IVM did not induce changes in the activity of antioxidant enzymes in A. thaliana rosettes, the expression of genes was significantly affected. Surprisingly, a higher number of transcripts, 300 and 438, respectively, was dysregulated in the rosettes than in roots. The significantly affected genes play role in response to salt, osmotic and water deprivation stress, in response to pathogens and in ion homeostasis. We hypothesize that the above described changes in gene transcription in A. thaliana resulted from disrupted ionic homeostasis caused by certain ionophore properties of IVM. Our results underlined the negative impact of IVM presence in the environment.
Permanent Link: http://hdl.handle.net/11104/0300329