PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION

0509659 - ÚEB 2020 RIV US eng M - Monography Chapter
Potocký, Martin - Bezvoda, R. - Pejchar, Přemysl
Antisense oligodeoxynucleotide-mediated gene knockdown in pollen tubes.
PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. TOTOWA: HUMANA PRESS INC., 2019 - (Cvrčková, F.; Žárský, V.), Roč. 1992 (2019), s. 359-365. Methods in Molecular Biology. ISBN 978-1-4939-9469-4
R&D Projects: GA ČR GA17-27477S; GA ČR(CZ) GC18-18290J; GA ČR(CZ) GA19-21758S
Institutional support: RVO:61389030
Keywords : Antisense oligodeoxynucleotide * Gene knockdown * Pollen tube * Tip growth
OECD category: Cell biology

Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Permanent Link: http://hdl.handle.net/11104/0300326