Photon-Upconversion Barcoding with Multiple Barcode Channels: Application for Droplet Microfluidics

0509591 - ÚIACH 2020 RIV US eng J - Journal Article
Hlaváček, Antonín - Křivánková, Jana - Přikryl, Jan - Foret, František
Photon-Upconversion Barcoding with Multiple Barcode Channels: Application for Droplet Microfluidics.
Analytical Chemistry. Roč. 91, č. 20 (2019), s. 12630-12635. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA ČR(CZ) GJ18-03367Y
Institutional support: RVO:68081715
Keywords : emission spectroscopy * droplet microfluidics * microfluidic chip * photon up conversions
OECD category: Analytical chemistry
Impact factor: 6.785, year: 2019
Method of publishing: Limited access

Barcoding facilitates high-throughput analytical methods in complex matrixes with a reduced volume of sample, reagents, time, and cost. Because of orthogonality to fluorescence, photon-upconversion barcodes attracted considerable attention in recent years. We constructed an epiluminescence detector, which, for the first time, demonstrated the reading of photon-upconversion spectra from microdroplets in a microfluidic chip with frequency up to 10 Hz. Non-negative least-squares deconvolution enabled the reading of an unprecedented number of photon-upconversion barcode channels (six) from emission spectra (excitation 980 nm, emission 430-875 nm). The standard deviation of barcode reading from microdroplets was ∼1%. Described barcoding can be, for example, used for multiparameter titrations, multiplexed biological and chemical assays, optimizations on a microfluidic platform, and preparation of barcoded concentration gradients and libraries.
Permanent Link: http://hdl.handle.net/11104/0300289