Seed germination, respiratory processes and phosphatidic acid accumulation in Arabidopsis diacylglycerol kinase knockouts – The effect of brassinosteroid, brassinazole and salinity

0509274 - ÚEB 2020 RIV US eng J - Journal Article
Derevyanchuk, M. - Kretynin, S. - Kolesnikov, Y. - Litvinovskaya, R. P. - Martinec, Jan - Khripach, V. - Kravets, V.
Seed germination, respiratory processes and phosphatidic acid accumulation in Arabidopsis diacylglycerol kinase knockouts – The effect of brassinosteroid, brassinazole and salinity.
Steroids. Roč. 147, JUL (2019), s. 28-36. ISSN 0039-128X. E-ISSN 1878-5867
R&D Projects: GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : Alternative oxidase * Arabidopsis thaliana * Brassinosteroids * Diacylglycerol kinase * Phosphatidic acid * Salinity
OECD category: Biochemical research methods
Impact factor: 1.948, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1016/j.steroids.2019.04.002

Using Arabidopsis thaliana wild type (WT)plants and diacylglycerol kinase knockouts (single mutants dgk3, dgk1, dgk6, double mutants dgk3dgk7, dgk5dgk6, dgk1dgk2)we observed that the inhibitor of brassinosteroid (BR)biosynthesis, brassinazole (BRZ), drastically decreased germination of dgk mutants under salt stress, while BRZ co-administration with 24-epibrassinolide (EBL)partially improved germination rates. We also observed a statistically significant decrease in alternative and cytochrome respiratory pathways in response to BRZ treatment under salinity conditions. We showed that production of the lipid second messenger phosphatidic acid (PA)is impaired in dgk mutants in response to EBL treatment and inhibitor of diacylglycerol kinase (DGK)– R59022. This study demonstrates that dgk mutants possess lower germination rates, lower total respiration rates, an alternative respiratory pathway and PA content under optimal and high salinity conditions in response to EBL treatment comparing to WT plants.
Permanent Link: http://hdl.handle.net/11104/0300030