Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis

0509088 - ÚIACH 2020 PL eng A - Abstract
Ryšavá, Lenka - Dvořák, Miloš - Miková, B. - Kubáň, Pavel
Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 20-20.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
R&D Projects: GA ČR(CZ) GA18-13135S
Institutional support: RVO:68081715
Keywords : amino acids * capillary electrophoresis * contactless conductivity detection
OECD category: Analytical chemistry

In this contribution, the process of sampling the DBSs and their subsequent elution with deionized water, methanol, acetonitrile, and optionally with mixtures thereof is shown. Various effects of the co-eluted matrix components on CE-C4D were observed for the selected elution solutions and are comprehensively described. The CE-C4D method was optimized for baseline separation of biogenic amino acids and indicated excellent analytical parameters and high stability of the separation system. CE-C4D was used for the direct analysis of the DBS eluates from healthy donors with amino acid concentrations at physiological levels. Finally, the method was applied to the determination of selected amino acids from DBS samples, which are related to common inborn metabolic disorders, such as maple syrup urine disease and phenylketonuria.
Permanent Link: http://hdl.handle.net/11104/0299877