Capillary electrophoresis and MALDI-TOF MS for rapid and reliable identification of viruses

0509071 - ÚIACH 2020 PL eng A - Abstract
Šalplachta, Jiří - Horká, Marie - Karásek, Pavel - Růžička, F. - Roth, Michal
Capillary electrophoresis and MALDI-TOF MS for rapid and reliable identification of viruses.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 9-9.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
R&D Projects: GA ČR(CZ) GA19-00742S; GA MV VI20172020069
Institutional support: RVO:68081715
Keywords : capillary electrophoretic (CE) * MALDI-TOF MS * complex biological samples * virus
OECD category: Analytical chemistry

The objective of this study is to demonstrate the ability of method combining CE in a capillary etched with supercritical water with MALDI-TOF MS for fast and reliable identification of viruses. The viruses were first dynamically adhered onto the roughened part of the separation capillary prior their on-line concentration and separation using tITP/ MEKC. The individual fractions were then collected from the capillary and off-line analyzed by MALDI-TOF MS.
Permanent Link: http://hdl.handle.net/11104/0299866