Accumulation of cyanobacterial oxadiazine nocuolin A is enhanced by temperature shift during cultivation and is promoted by bacterial co-habitants in the culture

0509023 - ÚVGZ 2020 RIV NL eng J - Journal Article
Chmelík, Dominik - Hrouzek, P. - Fedorko, Jan - Vu, D.L. - Urajová, P. - Mareš, J. - Červený, Jan
Accumulation of cyanobacterial oxadiazine nocuolin A is enhanced by temperature shift during cultivation and is promoted by bacterial co-habitants in the culture.
Algal Research-Biomass Biofuels and Bioproducts. Roč. 44, dec (2019), č. článku 101673. ISSN 2211-9264. E-ISSN 2211-9264
R&D Projects: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) LM2015055
Research Infrastructure: CzeCOS II - 90061
Institutional support: RVO:86652079
Keywords : axenization * secondary metabolite * nocuolin a, culture * culture * cyanobacteria
OECD category: Plant sciences, botany
Impact factor: 4.008, year: 2019
Method of publishing: Open access with time embargo
https://www.sciencedirect.com/science/article/pii/S2211926419302061?via%3Dihub

Proper setting of cultivation conditions is essential for production of high-value compounds in microbial biotechnology. The present study characterizes photoautotrophic growth and capacity to accumulate the antiproliferative secondary metabolite Nocuolin A (NoA) in cyanobacterium Nostoc sp. CCAP 1453/38. As the cyanobacterial culture was found to be non-axenic, the bacteria accompanying the culture were characterized, then the growth demands and NoA production in the Nostoc-bacterial consortium were determined, and finally an axenic strain was prepared. For the purposes of growth characterization, the culture was maintained in a quasi-continuous regime under various light intensities, temperatures, and inorganic carbon concentrations in a small-scale laboratory photobioreactor. The maximum biomass growth rate obtained was 0.10 h-1 (doubling time Dt = 6.93 h). As the temperature optima for the biomass production and for NoA accumulation differed, biphasic cultivation for maximal NoA yield was designed, leading to a three times more effective cultivation procedure compared to batch culture maintained at a temperature optimal for NoA production. The increased NoA accumulation at reduced temperature that correlated with enhanced expression of NoA biosynthetic genes after the temperature shift suggested its regulation occurs at the expression level. It has further been shown that NoA production is reduced in axenic culture, which indicates that it is also triggered by presence of bacteria. This study shows an example of how a biphasic cultivation mode with different temperatures can be used in high-value compound production processes. It also brings direct evidence that cyanobacterial strain axenization can lead to a rapid decrease in production of valuable compounds and that non-axenic strains may be considered more suitable for retrieval and initial production of novel pharmaceutical leads
Permanent Link: http://hdl.handle.net/11104/0300584