Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

0508882 - ÚIACH 2020 RIV DE eng J - Journal Article
Lačná, J. - Přikryl, Jan - Teshima, N. - Murakami, H. - Esaka, Y. - Foret, František - Kubáň, Petr
Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples.
Electrophoresis. Roč. 40, 18-19 (2019), s. 2390-2397. ISSN 0173-0835. E-ISSN 1522-2683
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * contactless conductivity detection * dual detection * laser induced fluorescence detection
OECD category: Analytical chemistry
Impact factor: 3.081, year: 2019
Method of publishing: Limited access

In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGEmay be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.
Permanent Link: http://hdl.handle.net/11104/0299680