Electrochemical analysis of glycoprotein samples prepared on a pneumatically-controlled microfluidic device

0508832 - ÚIACH 2020 RIV DE eng J - Journal Article
Novotný, Jakub - Ostatná, Veronika - Foret, František
Electrochemical analysis of glycoprotein samples prepared on a pneumatically-controlled microfluidic device.
Electroanalysis. Roč. 31, č. 10 (2019), s. 1994-2000. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR(CZ) GA18-18154S
Institutional support: RVO:68081715 ; RVO:68081707
Keywords : glycoprotein * microfluidics * square wave voltammetry
OECD category: Analytical chemistry; Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) (BFU-R)
Impact factor: 2.544, year: 2019
Method of publishing: Limited access

In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro-milling. The flow control and the fluid dosage into the micro-channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro-channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate-specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode.
Permanent Link: http://hdl.handle.net/11104/0299635