A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance

0508567 - BC 2020 RIV GB eng J - Journal Article
Wan, F. - Yin, C. - Tang, R. - Chen, M. - Wu, Q. - Huang, C. - Qian, W. - Rota-Stabelli, O. - Yang, N. - Wang, S. - Wang, G. - Zhang, G. - Guo, J. - Gu, L. - Chen, L. - Xing, L. - Xi, Y. - Liu, F. - Lin, K. - Guo, M. - Liu, W. - He, K. - Tian, R. - Jacquin-Joly, E. - Franck, P. - Siegwart, M. - Ometto, L. - Anfora, G. - Blaxter, M. - Meslin, C. - Nguyen, Petr - Dalíková, Martina - Marec, František - Olivares, J. - Maugnin, S. - Shen, J. - Liu, J. - Guo, J. - Luo, J. - Liu, B. - Fan, W. - Feng, L. - Zhao, X. - Peng, X. - Wang, K. - Liu, L. - Zhan, H. - Liu, W. - Shi, G. - Jiang, C. - Jin, J. - Xian, X. - Lu, S. - Ye, M. - Li, M. - Yang, M. - Xiong, R. - Walters, J. R. - Li, F.
A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance.
Nature Communications. Roč. 10, SEP 17 (2019), č. článku 4237. E-ISSN 2041-1723
Institutional support: RVO:60077344
Keywords : codling moth * genome sequence * genetic basis of invasiveness
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 12.121, year: 2019
Method of publishing: Open access
https://www.nature.com/articles/s41467-019-12175-9.pdf

The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.
Permanent Link: http://hdl.handle.net/11104/0300102