Effects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope

0508111 - ÚPT 2020 RIV NL eng J - Journal Article
Dordevic, B. - Neděla, Vilém - Tihlaříková, Eva - Trojan, V. - Havel, L.
Effects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope.
New Biotechnology. Roč. 48, JAN (2019), s. 35-43. ISSN 1871-6784. E-ISSN 1876-4347
R&D Projects: GA MŠMT(CZ) LO1212; GA ČR(CZ) GA14-22777S; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : copper * arsenic * somatic embryogenesis * Picea abies (L.) Karst. * environmental scanning electron microscope
OECD category: Plant sciences, botany
Impact factor: 4.674, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S1871678417305174?via%3Dihub

Somatic embryogenesis is an important biotechnological technique which can be used in studies associated with environmental stress. Four embryogenic cell lines of Norway spruce were grown on media enriched with copper and arsenic in concentration ranges 50-500 mu M and 10-50 mu M, respectively. The effects were observed during subsequent stages of somatic embryogenesis, the characteristics evaluated being proliferation potential, average number of somatic embryos obtained per g/fresh weight, morphology of developed somatic embryos, metal uptake, and microanalysis of macro-and micronutrients uptake. Copper and arsenic at higher concentrations significantly reduced the growth of early somatic embryos. In almost all treatments, the cell line V-1-3 showed the best performance compared with the other lines tested. Environmental scanning electron microscopy was used to visualize and identify morphological abnormalities in the development of somatic embryos. Abnormalities observed were classified into several categories: meristemless somatic embryos, somatic embryos with disrupted meristem, reduced number of cotyledons, single cotyledon and fused cotyledons. With the application of a low temperature method for the environmental scanning electron microscope, samples were stabilized and whole meristems could be investigated in their native state. As far as we are aware, this is the first report of the effect of copper and arsenic during the process of somatic embryogenesis and the first to evaluate the content of macro and micronutrients uptake in Norway spruce.
Permanent Link: http://hdl.handle.net/11104/0299099