Platforms for Single-Cell Collection and Analysis

0508038 - BTÚ 2020 RIV CH eng J - Journal Article
Valihrach, Lukáš - Androvič, Peter - Kubista, Mikael
Platforms for Single-Cell Collection and Analysis.
International Journal of Molecular Sciences. Roč. 19, č. 3 (2018), č. článku 807. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA16-10214S; GA ČR(CZ) GA17-04034S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : circulating tumor-cells * collection * isolation * analysis
OECD category: Biochemistry and molecular biology
Impact factor: 4.183, year: 2018
Method of publishing: Open access
https://www.mdpi.com/1422-0067/19/3/807/htm

Single-cell analysis has become an established method to study cell heterogeneity and for rare cell characterization. Despite the high cost and technical constraints, applications are increasing every year in all fields of biology. Following the trend, there is a tremendous development of tools for single-cell analysis, especially in the RNA sequencing field. Every improvement increases sensitivity and throughput. Collecting a large amount of data also stimulates the development of new approaches for bioinformatic analysis and interpretation. However, the essential requirement for any analysis is the collection of single cells of high quality. The single-cell isolation must be fast, effective, and gentle to maintain the native expression profiles. Classical methods for single-cell isolation are micromanipulation, microdissection, and fluorescence-activated cell sorting (FACS). In the last decade several new and highly efficient approaches have been developed, which not just supplement but may fully replace the traditional ones. These new techniques are based on microfluidic chips, droplets, micro-well plates, and automatic collection of cells using capillaries, magnets, an electric field, or a punching probe. In this review we summarize the current methods and developments in this field. We discuss the advantages of the different commercially available platforms and their applicability, and also provide remarks on future developments.
Permanent Link: http://hdl.handle.net/11104/0298997