0507955 - MBÚ 2020 RIV DE eng J - Journal Article
Němcová‐Fürstová, V. - Balušíková, K. - Halada, Petr - Pavlíková, N. - Šrámek, J. - Kovář, J.
Stearate-Induced Apoptosis in Human Pancreatic beta-Cells is Associated with Changes in Membrane Protein Expression and These Changes are Inhibited by Oleate.
Proteomics Clinical Applications. Roč. 13, č. 4 (2019), č. článku 1800104. ISSN 1862-8346. E-ISSN 1862-8354
Institutional support: RVO:61388971
Keywords : 2D electrophoresis * beta-cell apoptosis * membrane protein
OECD category: Biochemistry and molecular biology
Impact factor: 2.489, year: 2019 ; AIS: 0.716, rok: 2019
Method of publishing: Limited access
Result website:
https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800104DOI: https://doi.org/10.1002/prca.201800104

Purpose Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic beta-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. Experimental design Employing 2DE analysis and subsequent western blot confirmation, the differences in membrane/membrane-associated protein expression in human beta-cell line NES2Y are assessed during cell death induction by stearate and its inhibition by oleate. Results Induction of apoptosis by stearate is associated with significantly increased levels of Hsp90 beta, peroxiredoxin-1, and 14-3-3 gamma in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes are significantly inhibited by oleate co-application. No expression changes are detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 is significantly decreased after stearate application also in the whole cell lysate. Conclusions and clinical relevance Several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic beta-cells are identified. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3 gamma represent novel molecules related to the effect of fatty acids on beta-cell viability.
Permanent Link: http://hdl.handle.net/11104/0298922