Arsenolipids in the green alga Coccomyxa (Trebouxiophyceae, Chlorophyta)

0507941 - MBÚ 2020 RIV GB eng J - Journal Article
Řezanka, Tomáš - Nedbalová, L. - Barcyté, D. - Vítová, Milada - Sigler, Karel
Arsenolipids in the green alga Coccomyxa (Trebouxiophyceae, Chlorophyta).
Phytochemistry. Roč. 164, AUG 2019 (2019), s. 243-251. ISSN 0031-9422. E-ISSN 1873-3700
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971
Keywords : Coccomyxa elongata * Coccomyxa onubensis * Trebouxiophyceae
OECD category: Microbiology
Impact factor: 3.044, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0031942218304886?via%3Dihub

Lipid-like compounds containing a dimethylarsinoyl group, i.e. Me2As(O)-, have been identified by liquid chromatography/inductively coupled plasma mass spectrometry (LC/ICP-MS) and non-aqueous reversed-phase high-performance liquid chromatography (positive and/or negative high-resolution tandem electrospray ionization mass spectrometry (NARP-HPLC/HR-ESI+(-)-MS/MS) from three strains of green algae of the genus Coccomyxa (Trebouxiophyceae, Chlorophyta). The algae were cultivated in a medium containing 10 g arsenic/L, i.e. 133.5 mmol/L of Na2HAsO4 center dot 7H(2)O. After extraction by methyl-tert-butyl ether (MTBE), total lipids were analyzed by ICP-MS or ESI-MS without any further separation or fractionation. A total of 39 molecular species of arsenic triacylglycerols (AsTAG), 15 arsenic phosphatidylcholines (AsPC), 8 arsenic phosphatidylethanolamines (AsPE), 6 arsenic phosphatidylinositols (AsPI), 2 arsenic phosphatidylglycerols (AsPG) and 5 unknown lipids (probably ceramides) were identified. The structures of all molecular species were confirmed by tandem MS. Dry matter of the individual strains contained different amounts of total arsenolipids, i.e. C. elongata CCALA 427 (0.32 mg/g), C. onubensis (1.48 mg/g), C. elongate S3 (2.13 mg/g). On the other hand, there were only slight differences between strains in the relative abundances of individual molecular species. Possible biosynthesis of long-chain lipids with the end group Me2As(O) has also been suggested.
Permanent Link: http://hdl.handle.net/11104/0300703