Temporary heat stress suppresses PAMP-triggered immunity and resistance to bacteria in Arabidopsis thaliana

0507915 - ÚEB 2020 RIV US eng J - Journal Article
Janda, M. - Lamparová, Lucie - Zubíková, Alžběta - Burketová, Lenka - Martinec, Jan - Krčková, Zuzana
Temporary heat stress suppresses PAMP-triggered immunity and resistance to bacteria in Arabidopsis thaliana.
Molecular Plant Pathology. Roč. 20, č. 7 (2019), s. 1005-1012. ISSN 1464-6722. E-ISSN 1364-3703
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA AV ČR(CZ) L200381652
Institutional support: RVO:61389030
Keywords : Arabidopsis thaliana * flagellin sensing 2 receptor * flg22 * heat stress * PAMP-triggered immunity * Pseudomonas syringae * reactive oxygen species
OECD category: Biochemical research methods
Impact factor: 4.326, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1111/mpp.12799

Recognition of pathogen-associated molecular patterns (PAMPs) is crucial for plant defence against pathogen attack. The best characterized PAMP is flg22, a 22 amino acid conserved peptide from flagellin protein. In Arabidopsis thaliana, flg22 is recognized by the flagellin sensing 2 (FLS2) receptor. In this study, we focused on biotic stress responses triggered by flg22 after exposure to temporary heat stress (HS). It is important to study the reactions of plants to multiple stress conditions because plants are often exposed simultaneously to a combination of both abiotic and biotic stresses. Transient early production of reactive oxygen species (ROS) is a well-characterized response to PAMP recognition. We demonstrate the strong reduction of flg22-induced ROS production in A. thaliana after HS treatment. In addition, a decrease in FLS2 transcription and a decrease of the FLS2 presence at the plasma membrane are shown after HS. In summary, our data show the strong inhibitory effect of HS on flg22-triggered events in A. thaliana. Subsequently, temporary HS strongly decreases the resistance of A. thaliana to Pseudomonas syringae. We propose that short exposure to high temperature is a crucial abiotic stress factor that suppresses PAMP-triggered immunity, which subsequently leads to the higher susceptibility of plants to pathogens.
Permanent Link: http://hdl.handle.net/11104/0298886