Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing

0507904 - FZÚ 2020 RIV US eng J - Journal Article
Kratochvílová, Irena - Kopečná, Olga - Bačíková, Alena - Pagáčová, Eva - Falková, Iva - Follett, S.E. - Elliott, K.W. - Varga, K. - Golan, Martin - Falk, Martin
Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing.
Langmuir. Roč. 35, č. 23 (2019), s. 7496-7508. ISSN 0743-7463
R&D Projects: GA MŠMT EF16_019/0000760; GA MŠMT EF16_013/0001406; GA MŠMT(CZ) LO1409; GA ČR(CZ) GA15-05095S; GA ČR(CZ) GA16-12454S; GA MZd NV16-29835A
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760; OP VVV - SAFMAT(XE) CZ.02.1.01/0.0/0.0/16_013/0001406
Institutional support: RVO:68378271 ; RVO:68081707
Keywords : cryopreservation * chromatin condensation * cell nuclei membrane
OECD category: Biophysics; Physical chemistry (BFU-R)
Impact factor: 3.557, year: 2019
Method of publishing: Limited access
https://doi.org/10.1021/acs.langmuir.8b02742

The mechanisms underlying cell protection from cryoinjury are not yet fully understood. Recent biological studies have addressed cryopreserved cell survival but have not correlated the cryoprotection effectiveness with the impact of cryoprotectants on the most important cell structure, the nucleus, and the freeze/thaw process. We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. Namely, we investigated both higher-order chromatin structure and nuclear envelope integrity as possible markers of freezing and thawing processes. Moreover, we analysed in detail the relationship between nuclear envelope integrity, chromatin condensation, freeze/thaw processes in cells and cryopreservation efficiency for DMSO, glycerol, trehalose and antifreeze protein.
Permanent Link: http://hdl.handle.net/11104/0298867