Antibacterial silver-conjugated magnetic nanoparticles: design, synthesis and bactericidal effect

0507831 - ÚMCH 2020 RIV US eng J - Journal Article
Shatan, Anastasiia-Bohdana - Venclíková, Kristýna - Zasońska, Beata Anna - Patsula, Vitalii - Pop-Georgievski, Ognen - Petrovský, Eduard - Horák, Daniel
Antibacterial silver-conjugated magnetic nanoparticles: design, synthesis and bactericidal effect.
Pharmaceutical Research. Roč. 36, č. 10 (2019), s. 1-12, č. článku 147. ISSN 0724-8741. E-ISSN 1573-904X
R&D Projects: GA ČR(CZ) GA17-04918S
Institutional support: RVO:61389013 ; RVO:67985530
Keywords : antibacterial activity * magnetic nanoparticles * silica shell
OECD category: Polymer science; Physical geography (GFU-E)
Impact factor: 3.242, year: 2019
Method of publishing: Limited access
https://link.springer.com/article/10.1007%2Fs11095-019-2680-x

The aim was to design and thoroughly characterize monodisperse Fe3O4@SiO2-Ag nanoparticles with strong antibacterial properties, which makes them a candidate for targeting bacterial infections.The monodisperse Fe3O4 nanoparticles were prepared by oleic acid-stabilized thermal decomposition of Fe(III) oleate. The particles were coated with silica shell using a water-in-oil reverse microemulsion, involving hydrolysis and condensation of tetramethyl orthosilicate. Resulting Fe3O4@SiO2 particles were modified by (3-mercaptopropyl)trimethoxysilane to introduce 1.1 mmol SH/g. Finally, the Fe3O4@SiO2-SH nanoparticles were decorated with silver nanoclusters formed by reduction of silver nitrate with NaBH4. The particles were analyzed by FTIR, X-ray photoelectron and atomic absorption spectroscopy, dynamic light scattering and vibrating sample magnetometry. The antibacterial activity of the Fe3O4@SiO2 and Fe3O4@SiO2-Ag nanoparticles was tested against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria cultivated on Luria agar plates or in Luria broth. The superparamagnetic Fe3O4@SiO2-Ag nanoparticles (21 nm in diameter. Saturation magnetization 26 A∙m2/kg) were successfully obtained and characterized. Inhibitory and toxic effects against bacteria were documented by incubation of the Fe3O4@SiO2-Ag nanoparticles with Staphylococcus aureus and Escherichia coli. The combination of magnetic properties together with bactericidal effects is suitable for the disinfection of medical instruments, water purification, food packaging, etc.
Permanent Link: http://hdl.handle.net/11104/0298796