Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp.

0507822 - ÚOCHB 2020 RIV GB eng J - Journal Article
Kočendová, Jitka - Vaňková, Eva - Volejníková, Andrea - Nešuta, Ondřej - Buděšínský, Miloš - Socha, Ondřej - Hájek, Miroslav - Hadravová, Romana - Čeřovský, Václav
Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp.
FEMS Yeast Research. Roč. 19, č. 3 (2019), č. článku foz013. ISSN 1567-1356. E-ISSN 1567-1364
R&D Projects: GA TA ČR(CZ) TA04010638; GA MZd(CZ) NV16-27726A
Institutional support: RVO:61388963
Keywords : antifungal peptides * biofilm inhibition * biofilm eradication * Candida albicans * Candida tropicalis * vulvovaginal candidiasis
OECD category: Microbiology
Impact factor: 3.193, year: 2019
Method of publishing: Limited access
https://academic.oup.com/femsyr/article-abstract/19/3/foz013/5315757?redirectedFrom=fulltext

Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses of which are difficult to manage due to biofilm formation. This study aimed at developing novel non-toxic compounds active against Candida spp. biofilms. We synthesised analogues of natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms and elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic, antifungal and anti-biofilm activities of LL-III/43 and peptide VIII were then tested. LL-III/43 and VIII showed moderate cytotoxicity to HUVEC-2 cells and had comparable inhibitory activity against C. albicans and non-albicans spp. The lowest minimum inhibitory concentration (MIC90) of LL-III/43 was observed towards Candida tropicalis (0.8 mu M). That was 8-fold lower than that of antimycotic amphotericin B. Both peptides can be used to inhibit Candida spp. biofilm formation. Biofilm inhibitory concentrations (BIC50) ranged from 0.9 to 58.6 mu M and biofilm eradication concentrations (BEC50) for almost all tested Candida spp. strains ranged from 12.8 to 200 mu M. Also proven were the peptides' abilities to reduce the area colonised by biofilms, inhibit hyphae formation and permeabilise cell membranes in biofilms. LL-III/43 and VIII are promising candidates for further development as therapeutics against VVC.
Permanent Link: http://hdl.handle.net/11104/0298788