Actin depolymerization is able to increase plant resistance against pathogens via activation of salicylic acid signalling pathway

0507812 - ÚEB 2020 RIV GB eng J - Journal Article
Leontovyčová, Hana - Kalachova, Tetiana - Trdá, Lucie - Pospíchalová, Romana - Lamparová, Lucie - Dobrev, Petre - Malínská, Kateřina - Burketová, Lenka - Valentová, O. - Janda, Martin
Actin depolymerization is able to increase plant resistance against pathogens via activation of salicylic acid signalling pathway.
Scientific Reports. Roč. 9, JUL 18 (2019), č. článku 10397. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR GA17-05151S; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : AGE-RELATED RESISTANCE * NONHOST RESISTANCE * ARABIDOPSIS
OECD category: Biochemical research methods
Impact factor: 3.998, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1038/s41598-019-46465-5

The integrity of the actin cytoskeleton is essential for plant immune signalling. Consequently, it is generally assumed that actin disruption reduces plant resistance to pathogen attack. Here, we demonstrate that actin depolymerization induced a dramatic increase in salicylic acid (SA) levels in Arabidopsis thaliana. Transcriptomic analysis showed that the SA pathway was activated due to the action of isochorismate synthase (ICS). The effect was also confirmed in Brassica napus. This raises the question of whether actin depolymerization could, under particular conditions, lead to increased resistance to pathogens. Thus, we explored the effect of pretreatment with actin-depolymerizing drugs on the resistance of Arabidopsis thaliana to the bacterial pathogen Pseudomonas syringae, and on the resistance of an important crop Brassica napus to its natural fungal pathogen Leptosphaeria maculans. In both pathosystems, actin depolymerization activated the SA pathway, leading to increased plant resistance. To our best knowledge, we herein provide the first direct evidence that disruption of the actin cytoskeleton can actually lead to increased plant resistance to pathogens, and that SA is crucial to this process.
Permanent Link: http://hdl.handle.net/11104/0298780