Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes

0507731 - BFÚ 2020 RIV NL eng J - Journal Article
Trefulka, Mojmír - Černocká, Hana - Fojt, Lukáš - Paleček, Emil - Ostatná, Veronika
Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes.
Analytica Chimica Acta. Roč. 1067, AUG 27 2019 (2019), s. 56-62. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR(CZ) GA18-18154S
Institutional support: RVO:68081707
Keywords : glycosylation * strategies * proteins * oligosaccharides * polysaccharides
OECD category: Analytical chemistry
Impact factor: 5.977, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0003267019303782?via%3Dihub

Altered glycosylation is a universal feature of cancer cells and certain glycans are well-known markers of tumor progression. In this work we studied two glycan isomers, 2,3-sialyllactose (3-SL) and 2,6-sialyllactose (6-SL), frequently appearing in glycoproteins connected with cancer. A combination of square wave voltammetry and glycan modification with osmium(VI) N, N, N', N'-tetramethylethylenedi-amine (Os(VI) tem) allowed to distinguish between these regioisomers, since the 6-SL molecule can bind three Os(VI), while the 3-SL only two Os(VI) moieties, as experiments using capillary electrophoresis, inductively coupled plasma mass spectrometry and thin layer chromatography showed. A similar pattern of Os(VI)-modification was found for isomers of sialyl-N-acetyllactosamine and sialylgalactose. Covalent adducts of Os(VI) tem with glycans yielded three reduction voltammetric peaks. The ratio of peak I/peak II heights depends on the content of individual regioisomer in the sample. Our proposed approach allows the determination of isomer percentage representation in the mixture after one voltammogram recording. These results show a new appropriate method for the discrimination of glycan isomers containing terminal sialic acid important for distinguishing between cancerous and non-cancerous origin of biomarkers. (C) 2019 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0298707