Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta

0507659 - ÚBO 2020 RIV NL eng J - Journal Article
Stejskalová, K. - Jánová, E. - Horecký, C. - Horecká, E. - Václavek, P. - Hubálek, Zdeněk - Relling, K. - Cvanová, M. - D'Amico, G. - Mihalca, A. D. - Modrý, David - Knoll, A. - Horin, P.
Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta.
Molecular Biology Reports. Roč. 46, č. 4 (2019), s. 4453-4461. ISSN 0301-4851. E-ISSN 1573-4978
Institutional support: RVO:68081766 ; RVO:60077344
Keywords : West Nile virus * Horse * Restriction fragment length polymorphism * Microsatellite * SLC11A1 * TLR4 * MHC * NKR * Polymorphism
OECD category: Biochemistry and molecular biology; Veterinary science (BC-A)
Impact factor: 1.402, year: 2019
Method of publishing: Limited access
http://dx.doi.org/10.1007/s11033-019-04900-w

The West Nile virus (WNV) is a mosquito-borne flavivirus causing meningoencephalitis in humans and animals. Due to their particular susceptibility to WNV infection, horses serve as a sentinel species. In a population of Romanian semi-feral horses living in the Danube delta region, we have analyzed the distribution of candidate polymorphic genetic markers between anti WNV-IgG seropositive and seronegative horses. Thirty-six SNPs located in 28 immunity-related genes and 26 microsatellites located in the MHC and LY49 complex genomic regions were genotyped in 57 seropositive and 32 seronegative horses. The most significant association (p(corr)<0.0002) was found for genotypes composed of markers of the SLC11A1 and TLR4 genes. Markers of five other candidate genes (ADAM17, CXCR3, IL12A, MAVS, TNFA), along with 5 MHC class I and LY49-linked microsatellites were also associated with the WNV antibody status in this model horse population. The OAS1 gene, previously associated with WNV-induced clinical disease, was not associated with the presence of anti-WNV antibodies.
Permanent Link: http://hdl.handle.net/11104/0298641