D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain

0507478 - ÚMCH 2020 RIV US eng J - Journal Article
Pongrac, I. M. - Dobrivojević Radmilović, M. - Brkić Ahmed, L. - Mlinarić, H. - Regul, J. - Škokić, S. - Babič, Michal - Horák, Daniel - Hoehn, M. - Gajović, S.
D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain.
Cell Transplantation. Roč. 28, č. 5 (2019), s. 553-567. ISSN 0963-6897. E-ISSN 1555-3892
R&D Projects: GA MŠMT(CZ) LQ1604
EU Projects: European Commission(XE) 316120 - GLOWBRAIN
Institutional support: RVO:61389013
Keywords : neural stem cells * magnetic resonance imaging * brain
OECD category: Polymer science
Impact factor: 3.341, year: 2019
Method of publishing: Open access
https://journals.sagepub.com/doi/pdf/10.1177/0963689719834304

Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.
Permanent Link: http://hdl.handle.net/11104/0298801