Methinium colorimetric sensors for the determination of cholesterol sulfate in an aqueous medium

0507410 - ÚMG 2020 RIV CH eng J - Journal Article
Kejik, Z. - Havlík, M. - Bříza, T. - Kaplánek, R. - Dolenský, B. - Králová, Jarmila - Martásek, P. - Král, V.
Methinium colorimetric sensors for the determination of cholesterol sulfate in an aqueous medium.
Sensors and Actuators B - Chemical. Roč. 245, June (2017), s. 1032-1038. E-ISSN 0925-4005
R&D Projects: GA MŠMT(CZ) LQ1604
Institutional support: RVO:68378050
Keywords : Cholesterol sulfate * Sulfated lipids * Steroids * Chemical sensors * Methinium salts * Troger's bases
OECD category: Biochemistry and molecular biology
Impact factor: 5.667, year: 2017
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/abs/pii/S0925400517301909?via%3Dihub

Methinium colorimetric sensors 1 and 2 were studied for the determination of cholesterol sulfate by UV-Vis spectroscopy in 0.001 M NaCl (H2O:MeOH, 7:3), pH = 7.34. Both of the tested sensors displayed high affinity, excellent selectivity and sensitivity for cholesterol sulfate against other steroids (estrone sulfate, pregnenolone sulfate, cholesterol, cholic acid and taurocholic acid). The determined values of conditional binding constants, linear ranges and detection limits demonstrated the high usability of these sensors for the determination of cholesterol sulfate. For example, the detection limits of tested sensors I and 2 for the detection of cholesterol sulfate were 0.16 mu M (0.07 mg/L, R-2 = 0.9966) and 1.3 mu M (0.59 mg/L, R-2 = 0.9872), respectively. Our detection limit strongly indicates the potential usability of these sensors for the determination of biologically important levels of cholesterol sulfate. (C) 2017 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0298403