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Inducible protein stabilization system in Leishmania mexicana
- 1.0507397 - BC 2020 RIV NL eng J - Journal Article
Podešvová, L. - Huang, H. - Yurchenko, Vyacheslav
Inducible protein stabilization system in Leishmania mexicana.
Molecular and Biochemical Parasitology. Roč. 214, JUN 2017 (2017), s. 62-64. ISSN 0166-6851. E-ISSN 1872-9428
R&D Projects: GA ČR(CZ) GA16-18699S
Institutional support: RVO:60077344
Keywords : gene-expression * Leishmania mexicana * ecDHFR * Trimethoprim * Protein stabilization
OECD category: Developmental biology
Impact factor: 1.744, year: 2017
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0166685117300464?via%3Dihub
Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD. (C) 2017 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0298390
Number of the records: 1