Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells

0507370 - ÚEM 2020 RIV CZ eng C - Conference Paper (international conference)
Jiráková, Klára - Moskvin, Maksym - Horák, Daniel - Jendelová, Pavla
Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells.
Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. Ostrava: Tanger Ltd., 2018, s. 668-673. ISBN 9788087294819.
[International Conference on Nanomaterials - Research and Application (NANOCON) /9./. Brno (CZ), 18.10.2017-20.10.2017]
R&D Projects: GA MŠMT(CZ) LQ1604
Institutional support: RVO:68378041 ; RVO:61389013
Keywords : neural stem cells * nanoparticles * oxidative stress * ascorbic acid
OECD category: Neurosciences (including psychophysiology; Polymer science (UMCH-V)

Cells labelled with iron oxide nanoparticles (ION) can be tracked by magnetic resonance imaging (MRI) in several applications. However, various studies demonstrated toxicity and oxidative stress induction associated with nanoparticles exposure. We analysed biologic effects after the exposure of two types of iron oxide nanoparticles (with and without an antioxidative agent, an ascorbic acid) on human neural stem cells. The labelled cells in gel phantoms were detected in MRI and they showed decreased relaxation rates in comparison with control. ION slightly decreased cell proliferation in comparison with unlabelled cells, which was dependent on concentration and presence of ascorbic acid. None of the nanoparticle type showed negative effect on cell viability and both demonstrated minor effect on reactive oxygen species (ROS) formation. Unfortunately, ascorbic acid bound to nanoparticles did not show any effect on ROS attenuation. Cells exposed to both types of nanoparticles showed increased positivity for a phosphorylated form of H2AX a marker of double strand breaks. We showed that ION in low concentrations do not affect cell viability, but have negative effect on cells on DNA level. Their potential use for oxidative stress reduction is dependent on the concentration of ascorbic acid bound to the nanoparticles and this should be further increased.
Permanent Link: http://hdl.handle.net/11104/0298373