Time evolution of trapped single cell microorganism

0507073 - ÚPT 2020 RIV US eng C - Conference Paper (international conference)
Bernatová, Silvie - Samek, Ota - Obruča, S. - Šerý, Mojmír - Zemánek, Pavel - Márová, I.
Time evolution of trapped single cell microorganism.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. Proceedings of SPIE. Vol. 9711. Bellingham: SPIE, 2016, č. článku 971104. ISBN 978-1-62841-945-0. ISSN 0277-786X.
[Conference: Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues /9./. San Francisco (US), 16.02.2016-17.02.2016]
R&D Projects: GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : optical tweezers * Raman spectroscopy * 2D correlation analysis * external stimuli
OECD category: Microbiology

The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.
Permanent Link: http://hdl.handle.net/11104/0298345