Isotachophoretic determination of ketone bodies and lactate in cattle serum

0506796 - ÚIACH 2020 RIV CZ eng C - Conference Paper (international conference)
Vyskočilová, V. - Pechová, A. - Pantůčková, Pavla
Isotachophoretic determination of ketone bodies and lactate in cattle serum.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), s. 466-469. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
Institutional support: RVO:68081715
Keywords : cattle serum * lactate * ketone bodies * isotachophoresis
OECD category: Analytical chemistry
http://www.ce-ce.org/user_uploads/history/CECE2014_Brno/CECE_2014_proceedings_full.pdf

The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
Permanent Link: http://hdl.handle.net/11104/0297961