Electrochemical Detection of p53 Protein Interactions with Plasmid DNAs Modified with Cisplatin Using Immunoprecipitation at Magnetic Microbeads

0506734 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Pivoňková, Hana - Tichý, Vlastimil - Orság, Petr - Šebest, Peter - Fojta, Miroslav
Electrochemical Detection of p53 Protein Interactions with Plasmid DNAs Modified with Cisplatin Using Immunoprecipitation at Magnetic Microbeads.
XXXIV. Moderní elektrochemické metody sborník přednášek. Ústí nad Labem: Best servis, 2014 - (Navrátil, T.; Fojta, M.; Pecková, K.), Č. 2014 (2014), s. 126-130. ISBN 978-80-905221-2-1.
[Moderní Elektrochemické Metody /34./. Jetřichovice (CZ), 19.05.2014-23.05.2014]
Institutional support: RVO:68081707
Keywords : recognition * platinum * binding
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Antineoplastic drug [cis-diamminedichloroplatinum(II)] (cisplatin) forms covalent adducts with DNA. Cisplatin-modified DNA can be determined sensitively using square-wave voltammetry at mercury electrodes. Tumor suppressor protein p53 binds to DNA in different modes, including sequence-and structure-specific ones and these interactions are influenced by modification of the DNA with cisplatin. In this contribution we present a simple immunoprecipitation technique with magnetic beads, followed by voltammetric determination of recovered cisplatinated DNA, for the evaluation of p53 protein binding to DNAs containing various target sites differing in their proneness to being internally modified with the platinum complex.
Permanent Link: http://hdl.handle.net/11104/0297924