DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES

0506727 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Petr, Marek - Bažantová, Pavla - Adámik, Matěj - Kejnovská, Iva - Dvořáková, Zuzana - Vorlíčková, Michaela - Pečinka, Petr - Brázdová, Marie
DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), Č. 2014 (2014), s. 349-353. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
Institutional support: RVO:68081707
Keywords : DNA
OECD category: Analytical chemistry

In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent.
Permanent Link: http://hdl.handle.net/11104/0297919