P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE

0506725 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Haroniková, Lucia - Špaček, Jan - Fojta, Miroslav
P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), Č. 2014 (2014), s. 222-223. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
Institutional support: RVO:68081707
Keywords : DNA
OECD category: Analytical chemistry

In this work, we present a new qualitative approach of detection of PCR products using electrochemistry on pencil electrodes. PCR products with an incorporated biotin-labeled dNTP (dCTP in this study) are detected via conjugated streptavidinealkaline phospatase. 1-Naphthyl phosphate (1-NP), which is dephosphorylated by alkaline phosphatase to release 1-naphthol, was used as a substrate in electrochemical detection of the PCR product. Voltammetric measurement on disposable pencil electrode is a very cheap and easy to use method. The system was optimized for plasmid DNA PCR product with potential to detect human genome DNA products and possible application in gene deletion monitoring.
Permanent Link: http://hdl.handle.net/11104/0297918