ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA

0506723 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Adámik, Matěj - Holanová, L. - Navrátilová, Lucie - Nygrinova, J. - Pokorova, J. - Petr, Marek - Tichý, Vlastimil - Brázdová, Marie
ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA.
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. Brno: Institute of Analytical Chemistry AS CR, 2014 - (Foret, F.; Křenková, J.; Drobníková, I.; Guttman, A.; Klepárník, K.), Č. 2014 (2014), s. 114-117. ISBN 978-80-904959-2-0.
[CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./. Brno (CZ), 20.10.2014-22.10.2014]
Institutional support: RVO:68081707
Keywords : binding * p63
OECD category: Analytical chemistry

DNA-protein interactions of core domains of p53, p63 and p73, members of tumor suppressor p53 family, were investigated by multiple methods with regard to verifying the sequence specificity with which short target oligonucleotides/long DNA fragments can be recognized. The sequence specificity of core domains and wtp53 full protein binding to specific sequence in both types of DNA substrates was confirmed in solution, on surface and in gels by ELISA, slot-blot and EMSA.
Permanent Link: http://hdl.handle.net/11104/0297915