Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication

0506718 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Vítová, Lada - Fojta, Miroslav - Vespalec, Radim
Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication.
XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Srsenová Lenka - Best Servis, 2015 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2015 (2015), s. 272-274. ISBN 978-80-905221-3-8.
[Moderní elektrochemické metody /35./. Jetřichovice (CZ), 18.05.2015-22.05.2015]
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:68081707
Keywords : separation * oligonucleotides * nucleotides * capillary electrophoresis
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Effects of background electrolyte pH, composition and concentration on the electrophoretic transport of short oligodeoxyribonucleotides (ODNs) in fused silica capillaries have been investigated in the presented study. Mobilities and separation efficiencies of ODNs peaks varied with all three background electrolyte characteristics. Just changes in the background electrolyte characteristics and acid-base properties of ODNs are not sufficient for a systematic explanation of the observed variations. Despite the indicated complexity of ODNs electrophoretic behaviour, we show that very high separation efficiencies of ODNs peaks can be achieved in background electrolytes of physiological pH.
Permanent Link: http://hdl.handle.net/11104/0297910