Examples of Using of Electrochemical Detection at Pencil Graphite Electrode with Enzymatic Labeling for Analysis of Nucleotide Sequence

0506711 - BFÚ 2020 RIV CZ eng C - Conference Paper (international conference)
Plucnara, Medard - Haroniková, Lucia - Špaček, Jan - Havran, Luděk - Horáková, Petra - Pivoňková, Hana - Ecsin, E. - Erdem, A. - Fojta, Miroslav
Examples of Using of Electrochemical Detection at Pencil Graphite Electrode with Enzymatic Labeling for Analysis of Nucleotide Sequence.
XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Srsenová Lenka - Best Servis, 2015 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2015 (2015), s. 175-179. ISBN 978-80-905221-3-8.
[Moderní elektrochemické metody /35./. Jetřichovice (CZ), 18.05.2015-22.05.2015]
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:68081707
Keywords : dna hybridization * Enzymatic labeling * Alkaline phosphatase
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Many examples of utilization of enzymatic labeling for DNA sequence analysis has been described in literature so far. Some of them involve hybridization with complementary biotinylated probe, while others use incorporation of biotinylated nucleotides into DNA strand by DNA polymerases. Common approach is then binding of streptavidine-enzyme conjugates to biotin tags and incubation with substrate, which is converted to detectable product. Here, two recent applications using this principle are described for the detection of PCR amplicons and for SNP typing. Both techniques are combined with detection at pencil graphite electrodes.
Permanent Link: http://hdl.handle.net/11104/0297904